تریپان بلو (آبی)کد T6146 سیگما آلدریچ

General description

The trypan blue dye is an azo dye,^[5] derived from toluidine.^[4] It is soluble in water and has a molecular weight of 961 g/mol.^[5]

Application

Trypan blue has been used:
• in cell viability assay^[6]
• to determine the number of living and necrotic cells by staining with 0.2% trypan blue solution.^[2][3]
• to stain plant tissue in order to to visualize cell death^[3]

Packaging

5, 25, 100 g in glass bottle

Biochem/physiol Actions

The trypan blue dye is useful to differentiate the dead and live cells based on the cell′s ability to take up or exclude the dye. A viable cell is assumed to possess an intact cell membrane and hence excludes this dye and the cytoplasm appears clear. While on the other hand non-viable cells shows a blue cytoplasm.^[1]